Cadherin-based adhesion is a potential target for niche manipulation to protect hematopoietic stem cells in adult bone marrow.
نویسندگان
چکیده
Antibodies The following monoclonal antibodies (Abs) were used for flowcytometry and cell sorting: anti-c-Kit (2B8, BD Biosciences), -Sca-1 (E13-161.7, BD Biosciences), -CD4 (L3T4, BD Biosciences), -CD8 (53-6.72, BD Biosciences), -B220 (RA3-6B2, BD Biosciences), -TER-119, -Gr-1 (RB6-8C5, BD Biosciences), -Mac-1 (M1/70, BD Biosciences), -CD34 (RAM34, eBioscience), -CD31 (MEC13.3, BD Biosciences), and anti-ALCAM (Ohneda et al., 2001). A mixture of CD4, CD8, B220, TER-119, Mac-1, and Gr-1 was used as the lineage mix (Lin). The following Ab was used for immunocytochemistry: anti-β-catenin (clone 14, BD Biosciences)..
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ورودعنوان ژورنال:
- Cell stem cell
دوره 6 3 شماره
صفحات -
تاریخ انتشار 2010